Shaquille O'Neal strained his left knee in the opening minutes of Tuesday's game after he went up for an alley-oop pass from Dwyane Wade and fell on Chicago's Othella Harrington.
O'Neal stayed on the floor for several minutes holding his knee before being helped off the floor. X-rays were negative and he will be re-evaluated before Wednesday's game at Indiana.
Miami ended up losing to the Bulls in overtime by a final score of 105-101.
Ben Gordon hit five 3-pointers in the 4th quarter, finishing the contest with 29 points.