The Czech Republic beat Canada 103-101 in overtime in the semifinals of the Olympic qualifying tournament.
Tomas Satoransky, who scored 18 points, hit the game-winning jumper with 1.8 seconds left in overtime.
Blake Schilb led the Czechs with 31 points.
RJ Barrett lead Canada with 22 points, while Andrew Wiggins added 21 points.
Canada opened overtime with a 5-0 run, but were outscored 9-2 to end the game.
They will miss the Olympics for the fifth consecutive time.